당신은 주제를 찾고 있습니까 “pellet paint co precipitant – DNA Isolation Step 3: Precipitating the DNA“? 다음 카테고리의 웹사이트 https://ppa.charoenmotorcycles.com 에서 귀하의 모든 질문에 답변해 드립니다: ppa.charoenmotorcycles.com/blog. 바로 아래에서 답을 찾을 수 있습니다. 작성자 DNA Learning Center 이(가) 작성한 기사에는 조회수 49,782회 및 좋아요 326개 개의 좋아요가 있습니다.
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d여기에서 DNA Isolation Step 3: Precipitating the DNA – pellet paint co precipitant 주제에 대한 세부정보를 참조하세요
Jason Williams, DNA Learning Center, shows how to precipitate DNA.
For more information and for the laboratory protocol, visit DNA Barcoding 101 at http://www.dnabarcoding101.org/
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Pellet Paint® Co-Precipitant | 69049 – Merck Millipore
Pellet Paint® Co-Precipitant is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acs (McCormick 1995, …
Source: www.merckmillipore.com
Date Published: 8/27/2021
View: 5984
DNA “Pellet-Paint” coprecipitant or similar composition?
DNA “Pellet-Paint” coprecipitant or similar composition? Hello!, I want to see the DNA pellet, is there any dye or something to paint the pellet and …
Source: www.researchgate.net
Date Published: 12/25/2022
View: 6176
Pellet Paint® NF Co-precipitant from Merck – SelectScience
Pellet Paint® NF Co-Precipitant* is a non-fluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rap removal of BigDye™ …
Source: www.selectscience.net
Date Published: 2/22/2022
View: 812
PELLET PAINT CO-PRECIPITANT – Thomas Scientific
Home · Laboratory · Supplies · Nucleic Ac Analysis; PELLET PAINT CO-PRECIPITANT. PRODUCT AVAILABILITY: D you know you can view a product’s availability …
Source: www.thomassci.com
Date Published: 5/28/2021
View: 7856
MilliporeSigma™ Novagen™ Pellet Paint™ Co-Precipitant
MilliporeSigma – Novagen Pellet Paint Co-Precipitant – Compatible with most molecular biology procedures and is free of contaminating nucleic acs and …
Source: www.fishersci.com
Date Published: 10/19/2022
View: 5976
주제와 관련된 이미지 pellet paint co precipitant
주제와 관련된 더 많은 사진을 참조하십시오 DNA Isolation Step 3: Precipitating the DNA. 댓글에서 더 많은 관련 이미지를 보거나 필요한 경우 더 많은 관련 기사를 볼 수 있습니다.
주제에 대한 기사 평가 pellet paint co precipitant
- Author: DNA Learning Center
- Views: 조회수 49,782회
- Likes: 좋아요 326개
- Date Published: 2012. 5. 15.
- Video Url link: https://www.youtube.com/watch?v=y7kny3Xy4k4
Pellet Paint® Co-Precipitant
Overview
Pellet Paint® Co-Precipitant is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids (McCormick 1995, McCormick 1996). The two-minute precipitation uses just 2 µl per reaction and requires no low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink color. The pellet can be easily followed during washing steps and prevents losses during handling.
Most applications of PCR benefit from a clean-up step in which primers and other reactants are removed and the target DNA is concentrated (Taggart 1998). Pellet Paint Co- Precipitant is ideal for this cleanup because the procedure is rapid, primers < 50 nt in length are efficiently removed, and the DNA is quantitatively recovered. Furthermore, it is easy to tell when the DNA has been completely resuspended following the precipitation step. Pellet Paint Co-Precipitant is compatible with most molecular biology procedures and is free of contaminating nucleic acids and nucleolytic enzymes. Although it absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package) can be used as a correction factor when determining nucleic acid concentration (McCormick 1996). Pellet Paint Co-Precipitant is compatible with automatted Cy5® sequencers. Pellet Paint NF Co- Precipitant is recommended for use with PE Applied Biosystems automated sequencers.
Pellet Paint® NF Co-precipitant from Merck
Pellet Paint® NF Co-Precipitant* is a non-fluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye Terminators (PE Corp.) during the alcohol precipitation of cycle sequencing reaction products.
Cycle sequencing reactions can be precipitated rapidly with centrifugation times of 10 minutes. The easily visualized carrier provides a simple confirmation that precipitation has occurred. Sequencing reaction products are efficiently pelleted and dye-labeled terminators remain in the supernatant during alcohol precipitation using the standard Applied Biosystems precipitation protocols. Resuspension of pelleted sequencing reaction products in deionized formamide can be confirmed by checking for dissolution of the carrier pellet.
Pellet Paint NF Co-Precipitant is fully compatible with the ABI PRISM® BigDye Terminator Cycle Sequencing Ready Reaction. To avoid extra sample handling, Pellet Paint NF Co-Precipitant can be added directly to the reaction mix, template DNA, crude PCR samples, or dilution buffer prior to the cycle sequencing reaction.
Although Pellet Paint absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package of Pellet Paint) can be used as a correction factor when determining nucleic acid concentration.
Pellet Paint NF Co-Precipitant has no detectable effect on the sequencing reaction or sequence accuracy. Pellet Paint NF is a useful substitute for the original Pellet Paint in applications where fluorescent detection is used.
Features
Efficient and rapid precipitation of BigDye cycle sequencing products
Efficient removal of dye terminators
Direct visualization and tracking of precipitated material
No effect on sequencing reaction
Substitute for original Pellet Paint Co-Precipitant for fluorescent detection applications
* patent pending
PELLET PAINT CO-PRECIPITANT
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MilliporeSigma Novagen Pellet Paint Co-Precipitant 100rxn:Molecular Biology
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키워드에 대한 정보 pellet paint co precipitant
다음은 Bing에서 pellet paint co precipitant 주제에 대한 검색 결과입니다. 필요한 경우 더 읽을 수 있습니다.
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사람들이 주제에 대해 자주 검색하는 키워드 DNA Isolation Step 3: Precipitating the DNA
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주제에 대한 기사를 시청해 주셔서 감사합니다 DNA Isolation Step 3: Precipitating the DNA | pellet paint co precipitant, 이 기사가 유용하다고 생각되면 공유하십시오, 매우 감사합니다.
